A cell-permeable sulfonyl acrylonitrile compound that acts as a potent and irreversible inhibitor of protein phosphatase methylesterase-1 (PME-1, IC50=600nM and 2.5uM in human cell lysates and in HEK 293T cells, (respectively) with 100-fold greater selectivity over other serine hydrolases, does not function as a general thiol-reactive probe. Shown to lower demethylated PP2A levels by ~80% at 20uM with elevated methylated PP2A (protein phosphatase 2A) form in PME-1 overexpressing HEK 293T cells. Synonyms: (E)-2-(4-Fluorophenylsulfonyl)-3-(1-(3-nitrophenylsulfonyl)-1H-pyrrol-2-yl)-acrylonitrile, NIH Probe ML136 Molecular Formula: C19H13FN3O6S2 Molecular Weight: 462.6 Solubility: DMSO (50mg/ml) Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
462.6
Purity:
97% (HPLC)
Form:
Supplied as a yellow solid.
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