Spautin-1 (6-fluoro-N-[4-fluorobenzyl]quinazolin-4-amine, Specific and Potent AUtophagy Inhibitor 1, C43, Autophagy Inhibitor II)

Product Overview

• Article Name: Spautin-1 (6-fluoro-N-[4-fluorobenzyl]quinazolin-4-amine, Specific and Potent AUtophagy Inhibitor 1, C43, Autophagy Inhibitor II)
• Biozol Catalog Number: USB-217803
• Supplier Catalog Number: 217803
• Alternative Catalog Number: USB-217803-10
• Manufacturer: US Biological
• Category: Biochemikalien

Product Description

A quinazolin compound that acts as a specific and potent inhibitor of autophagy and promotes Vps34 PI 3-kinase complex degradation by blocking the activity of USP 10 (IC50 = 580nM) and USP 13 (IC50 = 690nM) deubiquitinating enzymes. However, it does not affect the lipid kinase activity of Vps34. Also shown to reduce the level of cytoplasmic and nuclear p53. Sensitizes breast cancer cell lines (BCAP37, MCF-7, and BT549) to apoptosis under conditions of nutritional deprivation. Also shown to inhibit etoposide-induced cell death in Bax/Bak double knockout (DKO) cells. Normal cell lines (e.g., MDCK cells) do not undergo autophagy in the presence of Spautin-1. Formula: C15H11F2N3 Molecular Weight: 371.3 Solubility: DMSO (50mg/ml, clear, colorless solution) Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 371.3
• Purity: 99% (HPLC)
• Form: Supplied as an off-white solid.