A potent, 2H-chromen-2-one small molecule inhibitor that is selective for TOP2 (IC50=2.8uM and 0.42uM for TOP2a and TOP2b, respectively). Unlike TOP2 poisons such as etoposide, it does not lead to DNA damage associated with DNA Double-Strand Breaks, as evidenced by the lack of y-H2AX accumulation but instead inhibits kinetoplast DNA decatenation in vitro. This compound affects cell cycle at the M phase and disturbs mitotic spindle formation in HeLa cells at 3-10uM. It also exhibits significant anti-proliferative activities against several human cancer cell lines. Molecular Formula: C24H25NO5 Molecular Weight: 407.46 Purity (HPLC): 98% Appearance: White solid Solubility: DMSO Method of Determining Identity: NMR and LCMS Spectometry Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
407.46
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