Ala-Ala-Phe-pNA is a synthetic peptide used in the study of proteolytic enzymes for its ability to mimic natural peptide bonds, making it an ideal choice for enzyme assays and screening applications. The incorporation of a p-nitroanilide moiety allows for easy spectrophotometric detection, enhancing its utility in laboratory settings. Synonyms: Ala-Ala-Phe p-nitroanilide, (2S)-2-[((2S)-2-{[(2S)-2-aminopropanoyl]amino}propanoyl)amino]-N-(4-nitrophenyl)-3-phenylpropanamide CAS No: 61043-41-2 Molecular Formula: C21H25N5O5 Molecular Weight: 427.46 Purity: 99% (Assay), 98% (TLC) Appearance: White to off-white powder Purity: 99% (Assay), 98% (TLC) TLC Conditions: Ethylacetate/ pyridine/ acetic acid/ water: (60/20/6/11) Chloroform/ methanol/ acetic acid (98-100%)/ water: (45/13/1/2) Chloroform/ methanol/ toluene/ water: 8/8/8/1 Plate: silicagel 60 F254 Detected by: UV, ninhydrin, chlorine-tolidine Melting Point: 201-207C (lit) Optical Rotation: [a]D20 = + 32 2 (C=0.5 in DMF) Related Substances: 0.1% (pNA) Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at 4C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight:
427.46
CAS Number:
[61043-41-2]
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