In cryobiology DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used. Synonyms: Methyl Sulfoxide, Dimethylsulfoxide, DMSO CAS No: 67-68-5 Molecular Formula: C2H6SO Molecular Weight: 78.13 Purity GC): 99.9% Appearance: Colorless, clear liquid at RT Solubility: Water Density: As Reported Water Content (KF): 0.1% Refractive Index (n20/D): 1.4755-1.4780 Absorbance: To Pass ACS Specifications pKa: 31-32 Acid Value: As Reported Titratable Acid: 0.001 meq/g Residue after Evaporation: 0.01% Congealing Temp: 18.3C Note: DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state.
Molecular Weight:
78.13
CAS Number:
[67-68-5]
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