| Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites. Synonyms: C.I. Direct Blue 14, Tetrasodium Salt, Diphenyl Blue, Amanil Sky Blue R, Bencidal Blue 3B, Benzamine Blue, Benzanil Blue 3BN, Benzo Blue 3BS, Benzo Blue, Blue 3B, Blue EMB, Brasilamina Blue 3B, C.I. 23850, C.I. Direct Blue 14, Chloramine Blue 3B, Chrome Leather Blue 3B, Congo Blue, Cresotine Blue 3B, Diamine Blue 3B, Diamineblue, Diaphtamine Blue TH, Diazine Blue 3B, Diazol Blue 3B, Diphenyl Blue 3B, Direct Blue 14, Direct Blue 3B, Direct Blue M 3B, Hispamin Blue 3BX, Naphthylamine Blue, Niagara Blue, Niagara Blue 3B, Paramine Blue 3B, Pontamine Blue 3BX, Sodium Ditolyldisazobis-8-amino-1-naphthol-3,6-disulfonate, Trypan (Congo) Blue, Trypan Blue BPC, Trypane Blue, 3,3-[(3,3-Dimethyl[1,1-biphenyl]-4,4-diyl)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonic Acid Tetrasodium CAS Number: 72-57-1 Molecular Formula: C34H24N6O14S4Na4 Molecular Weight: 960.82 Purity (Dye content): 70% Appearance: Black powder UV Ratios: lambdamax in methanol: 605 2 Absorptivity (1%/1cm) at lambdamax 603.0nm in methanol: 625 Melting Point: >300C (lit) Solubility: Blue solution (C=0.1% in water) Loss on Drying: 10% (110C/1 hour) Storage and Stability: Powder may be stored at RT. Stable for 6 months at RT after receipt. Warnings! Cancer suspect agent! Teratogen! Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures. |
