Resorufin a-D-glucopyranoside, CAS [[136565-93-3]]

Product Overview

• Article Name: Resorufin a-D-glucopyranoside, CAS [[136565-93-3]]
• Biozol Catalog Number: USB-288989
• Supplier Catalog Number: 288989
• Alternative Catalog Number: USB-288989-1,USB-288989-5
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Resorufin alpha-D-glucopyranoside is a sensitive fluorogenic substrate that generates a red fluorescent product (resorufin) upon interaction with alpha-glucosidase. It is used for measuring alpha-glucosidase activities and high throughput screening of alpha-glucosidase inhibitors. Alpha-glucosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-glucolactosyl moieties from glycolipids and glycoproteins. Mutations in alpha-glucosidase cause accumulation of glycogen in lysosomes, resulting in Pompe disease, a lysosomal storage disorder. Small molecule chaperones that bind to enzyme proteins and correct the misfolding and mistrafficking of mutant proteins have emerged as a new therapeutic approach for the lysosomal storage disorders. In addition, alpha-glucosidase is a therapeutic target for type II diabetes, and alpha-glucosidase inhibitors have been used in the clinic as alternative treatments for this disease. Resorufin alpha-D-glucopyranoside is a new fluorogenic substrate for the alpha-glucosidase enzyme assay, resorufin alpha-d-glucopyranoside. The enzyme reaction product of this new substrate emits at a peak of 590nm, reducing the interference from fluorescent compounds seen with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-glucopyranoside. Also, the enzyme kinetic assay can be carried out continuously without the addition of stop solution due to the lower pK(a) of the product of this substrate. Therefore, this new fluorogenic substrate is a useful tool for the alpha-glucosidase enzyme assay and will facilitate compound screening for the development of new therapies for Pompe disease. Synonym: 3H-Phenoxazin-3-one, 7-(.alpha.-D-glucopyranosyloxy)-, Resorufin alpha-D-glucopyranoside CAS No: 136565-93-3 Molecular Formula: C18H17NO8 Molecular Weight: 375.33 Appearance: Red solid Purity: 95% (HPLC) Excitation (nm): 570 Emission (nm): 583 Extinction Coefficient (cm-1 M-1): 65,000 Quantum Yield: 0.75 Storage and Stability: May be stored at 4C. Light sensitive, amber vial. Stable for 6 months at RT after receipt

Product Properties

• Molecular Weight: 375.33
• Purity: 95% (HPLC)
• Form: Red solid
• CAS Number: [136565-93-3]