BRD3 Inhibitor Screening, BioAssay(TM) Kit (BD2, RING3L, Bromodomain)

Product Overview

• Article Name: BRD3 Inhibitor Screening, BioAssay(TM) Kit (BD2, RING3L, Bromodomain)
• Biozol Catalog Number: USB-298357
• Supplier Catalog Number: 298357
• Alternative Catalog Number: USB-298357-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The BRD3 (BD2) Inhibitor Screening BioAssay(TM) Kit is designed to measure the inhibition of BRD3 bromodomain 2 (BD2) from binding to its substrate. The key to the BRD3 (BD2) Inhibitor Screening BioAssay(TM) Kit is the highly specific binding of the BRD3 bromodomain 2 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD3 bromodomain 2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts. Applications: Suitable for use in the study of bromodomain binding assays, screening inhibitors and selectivity profiling. Other applications not tested. Kit Components: BRD3 (306-417), BD2, GST-tag, 1x20ug BET Bromodomain Ligand, 1x400ul Non-acetylated Ligand, 1x200ul 3x BRD Homogeneous Assay Buffer 1, 1x4ml 3x BRD Homogeneous Detection Buffer 1, 1x3ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.