MTOR/Raptor/MLST8 Complex, Recombinant, Human, FLAG-tag, His-Tag (Mechanistic Target of Rapamycin, FRAP, FKBP-Rapamycin Associated Protein, Mammalian Target Of Rapamycin, Regulatory Associated Protein of MTOR, Mip1, MLST8, Mammali
Catalog Number:
USB-298432
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| Article Name: | MTOR/Raptor/MLST8 Complex, Recombinant, Human, FLAG-tag, His-Tag (Mechanistic Target of Rapamycin, FRAP, FKBP-Rapamycin Associated Protein, Mammalian Target Of Rapamycin, Regulatory Associated Protein of MTOR, Mip1, MLST8, Mammali |
| Biozol Catalog Number: | USB-298432 |
| Supplier Catalog Number: | 298432 |
| Alternative Catalog Number: | USB-298432-10 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]. Source: Recombinant protein corresponding to human MTOR/Raptor/MLST8 Complex, fused to FLAG-tag (mTOR) and His-tag (Raptor/MLST8) at N-terminal, expressed in Sf9 insect cells. Molecular Weight: ~324kD (complex) Specific Activity: 7.1pmol/min/ug Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Assay Conditions: Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM MgCl2, 1mM EGTA, 200um ATP, 0.01% Brij-35 and 2um substrate (Zlyte Ser/Thr peptide 11) at RT for 1 hour. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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