PCSK9 (D374T), Recombinant, Human, aa31-692 (Proprotein Convertase Subtilisin/Kexin Type 9, FH3, HCHOLA3, PC9) (Biotin)
Catalog Number:
USB-298444
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| Article Name: | PCSK9 (D374T), Recombinant, Human, aa31-692 (Proprotein Convertase Subtilisin/Kexin Type 9, FH3, HCHOLA3, PC9) (Biotin) |
| Biozol Catalog Number: | USB-298444 |
| Supplier Catalog Number: | 298444 |
| Alternative Catalog Number: | USB-298444-25 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| PCSK9 is a crucial player in the regulation of plasma cholesterol homeostasis. It binds to the ectodomain of hepatic low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation. PCSK9 acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. The D374T mutation results in higher affinity of PCSK9 for LDLR. Source: Recombinant protein corresponding to aa31-692 from human PCSK9, fused to C-terminal His- and Avi-tags and a D374T mutation, expressed in HEK293 cells. Molecular Weight: ~74kD, PCSK9 is autocleaved to the ~14kD prodomain (aa31-152) and the ~60kD mature form (aa153-692), which run at higher MW by SDS-PAGE. Applications: Suitable for use in the study of enzyme kinetics, binding studies, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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