Bcl is an anti-apoptic protein. It has been implicated in a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. It is also thought to be involved in resistance to conventional cancer treatment. This supports a role for decreased apoptosis in the pathogenesis of cancer. Source: Recombinant protein corresponding to aa1-218 of human Bcl-2, fused to His-tag, expressed in E. coli. Applications: Suitable for use as an Input marker or positive control for Western Blot and for Function studies (binding assays). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
95% (RP-HPLC, SDS-PAGE)
Form:
Supplied as a lyophilized powder in 10mM Tris-HCl, pH 8.0, 1mM EDTA, 250mM sodium chloride. Reconstitute with 100ul of 10% acetic acid (Glacial).
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