This protein may be used in functional toxicity assays in combination with rLukD, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus. Purified, Staphylococcus aureus recombinant leukocidin-E (rLukE). The rLukE (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 34,819 Daltons. Molecular Weight: ~35kD Applications: Suitable for use in ELISA, Western Blot and Cytotoxicity assay. Other applications not tested. Recommended Dilutions: Western Blot: 100ng Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
35
Purity:
Purified.
Form:
Supplied as a liquid in PBS, 5% glycerol.
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