Orexin-A and B were identified as endogenous ligands for the orexin-1 (HCRT1) and orexin-2 (HCRT2) G-protein coupled receptors (Sakurai et al, 1998). These peptides are identical to two hypothalamic peptides that share a high degree of homology with secretin, designated hypocretin-1 and hypocretin-2 (De Lecea et al, 1998). ICV administration of orexin-A and B stimulated food intake in rats. Additionally, prepro-orexin mRNA levels were upregulated in fasting rats. Recent reports suggest a role in the sleep-wake cycle. Molecular Weight: ~2957.4 Sequence: Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly -Ile-Leu-Thr-Met-Gly Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
2957.4
Purity:
~95% (HPLC)
Form:
Supplied as a white powder.
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