ATP1B2 is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-2 subunit is not known. The protein is composed of three subunits: alpha (catalytic), beta and gamma. Source: Recombinant protein corresponding to aa68-290 of human ATP1B2, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~27.8kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDHTPKYQ DRLATPGLMI RPKTENLDVI VNVSDTESWD QHVQKLNKFL EPYNDSIQAQ KNDVCRPGRY YEQPDNGVLN YPKRACQFNR TQLGNCSGIG DSTHYGYSTG QPCVFIKMNR VINFYAGANQ SMNVTCAGKR DEDAENLGNF VMFPANGNID LMYFPYYGKK FHVNYTQPLV AVKFLNVTPN VEVNVECRIN AANIATDDER DKFAGRVAFK LRINKT Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
