CCS, Recombinant, Human, aa1-274, His-Tag (Copper Chaperone for Superoxide Dismutase)

Product Overview

• Article Name: CCS, Recombinant, Human, aa1-274, His-Tag (Copper Chaperone for Superoxide Dismutase)
• Biozol Catalog Number: USB-349764
• Supplier Catalog Number: 349764
• Alternative Catalog Number: USB-349764-100, USB-349764-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

CCS is essential for the incorporation of copper into SOD-1, and therefore is necessary for its enzymatic activity. CCS prevents copper ions from binding to intracellular copper scavengers and provides the SOD-1 enzyme with the necessary copper cofactor. CCS escorts copper only to SOD-1 and fails to deliver copper to proteins in the mitochondria, nucleus or secretory pathway. While many tissues express CCS, the chaperone is most abundant in the kidney, liver and Purkinje cells in the neuropil of the central nervous system. Recombinant protein corresponding to aa1-274 of human CCS, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~31.2kD (MALDI-TOF) Amino Acids Sequence: MGSSHHHHHH SSGLVPRGSH MASDSGNQGT LCTLEFAVQM TCQSCVDAVR KSLQGVAGVQ DVEVHLEDQM VLVHTTLPSQ EVQALLEGTG RQAVLKGMGS GQLQNLGAAV AILGGPGTVQ GVVRFLQLTP ERCLIEGTID GLEPGLHGLH VHQYGDLTNN CNSCGNHFNP DGASHGGPQD SDRHRGDLGN VRADADGRAI FRMEDEQLKV WDVIGRSLII DEGEDDLGRG GHPLSKITGN SGERLACGII ARSAGLFQNP KQICSCDGLT IWEERGRPIA GKGRKESAQP PAHL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 31.2
• NCBI: 005116
• UniProt: O14618
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM DTT, 10% glycerol.