CHRNA3 also known as neuronal acetylcholine receptor subunit alpha-3 isoform 1 is a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. CHRNA3 is a ligand-gated ion channel that likely plays a role in neurotransmission. CHRNA3 have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. Source: Recombinant protein corresponding to aa32-240 of human CHRNA3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~27kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSEAEHRL FERLFEDYNE IIRPVANVSD PVIIHFEVSM SQLVKVDEVN QIMETNLWLK QIWNDYKLKW NPSDYGGAEF MRVPAQKIWK PDIVLYNNAV GDFQVDDKTK ALLKYTGEVT WIPPAIFKSS CKIDVTYFPF DYQNCTMKFG SWSYDKAKID LVLIGSSMNL KDYWESGEWA IIKAPGYKHD IKYNCCEEIY PDITYSLYIR RL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
