CysH, Recombinant, E. coli, aa1-244, His-Tag (Phosphoadenosine phosphosulfate reductase)

Catalog Number: USB-349836
Article Name: CysH, Recombinant, E. coli, aa1-244, His-Tag (Phosphoadenosine phosphosulfate reductase)
Biozol Catalog Number: USB-349836
Supplier Catalog Number: 349836
Alternative Catalog Number: USB-349836-100, USB-349836-500
Manufacturer: US Biological
Category: Molekularbiologie
CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3,5-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3-phosphoadenylyl sulfate and thioredoxin. Source: Recombinant protein corresponding to aa1-244 of E. coli CysH, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~30.1kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 30.1
NCBI: 417242
Purity: 90% (SDS-PAGE)
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 50mM sodium chloride, 10% glycerol.