DHODH, also known as dihydroorotate dehydrogenase (quinone), mitochondrial, belongs to the dihydroorotate dehydrogenase family. DHODH catalyzes the fourth enzymatic step, the ubiquinone- mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Source: Recombinant protein corresponding to aa31-395 of human DHODH, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~42.3kD AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTGDER GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP IDGLIVTNTT VSRPAGLQGA LRSETGGLSG VGKVKRELEA LLKEQGFGGV TDAIGADHRR FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
