DsbE, Recombinant, E. coli, aa26-185 (Thiol:disulfide interchange protein dsbE)
Biozol Catalog Number:
USB-349859
Supplier Catalog Number:
349859
Alternative Catalog Number:
USB-349859-100, USB-349859-500
Manufacturer:
US Biological
Category:
Molekularbiologie
DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Source: Recombinant protein corresponding to aa26-185 of E. coli DsbE, expressed in E. coli. Molecular Weight: ~18.1kD Amino Acid Sequence: MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
