EEF1E1, also known as eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Source: Recombinant protein corresponding to aa1-174 of human EEF1E1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~21.9kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
