Map, Recombinant, E. coli, aa1-264, His-Tag (Methionine aminopeptidase)

Catalog Number: USB-350057
Article Name: Map, Recombinant, E. coli, aa1-264, His-Tag (Methionine aminopeptidase)
Biozol Catalog Number: USB-350057
Supplier Catalog Number: 350057
Alternative Catalog Number: USB-350057-100,USB-350057-500
Manufacturer: US Biological
Category: Molekularbiologie
Methionine aminopeptidases, also designated peptidase M proteins, are members of the M24 family of proteins. This protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of map contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Source: Recombinant protein corresponding to aa1-264 from E. coli Map, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~31.5kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 31.5
NCBI: 414710
Purity: 90% (SDS-PAGE)
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 10% glycerol.