NPL, also known as N-acetylneuraminate lyase, is an enzyme that catalyzes the chemical reaction. (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate) This protein belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. It participates in amino sugars metabolism. Source: Recombinant protein corresponding to human NPL protein, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~37.3kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAFPKKKLQG LVAATITPMT ENGEINFSVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSV SERRQVAEEW VTKGKDKLDQ VIIHVGALSL KESQELAQHA AEIGADGIAV IAPFFLKPWT KDILINFLKE VAAAAPALPF YYYHIPALTG VKIRAEELLD GILDKIPTFQ GLKFSDTDLL DFGQCVDQNR QQQFAFLFGV DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDFS LALNYQFCIQ RFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDSAEAKL KSLDFLSFTD LKDGNLEAGS Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
