POLR3F, also known as RPC39 and RPC6, belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). It is DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Source: Recombinant protein corresponding to aa1-316 of human POLR3F, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~38.1kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQRN SSFASSHEVW KYICELGISK VELSMEDIET ILNTLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
