RDBP, also known as NELF-E, is a putative RNA binding protein. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions. Source: Recombinant protein corresponding to aa1-380 from human RDBP, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~45.4kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLVIPPGLSE EEEALQKKFN SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
