RHOA, Recombinant, Human, aa1-190, His-Tag

Product Overview

• Article Name: RHOA, Recombinant, Human, aa1-190, His-Tag
• Biozol Catalog Number: USB-350239
• Supplier Catalog Number: 350239
• Alternative Catalog Number: USB-350239-100,USB-350239-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila). Recombinant protein corresponding to aa1-190 of human RHOA, fused to His-Tag at N-terminal expressed in E. coli. Molecular Weight: ~25.7kD (MALDI-TOF) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 25.7
• NCBI: 001655
• UniProt: P61586
• Purity: 90% (SDS-PAGE). Purified by using conventional chromatography techniques.
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol.