RnhA, Recombinant, E. coli, aa1-155, His-Tag (Ribonuclease HI, degrades RNA of DNA-RNA hybrids)
Biozol Catalog Number:
USB-350244
Supplier Catalog Number:
350244
Alternative Catalog Number:
USB-350244-100,USB-350244-500
Manufacturer:
US Biological
Category:
Molekularbiologie
rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5-phosphomonoester and is able to bind magnesium or manganese as cofactors. . Source: Recombinant protein corresponding to aa1-155 of E. coli rnhA, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~20kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
