TBCC, Recombinant, Human, aa1-346, His-Tag (Tubulin-specific chaperone C)
Biozol Catalog Number:
USB-350321
Supplier Catalog Number:
350321
Alternative Catalog Number:
USB-350321-20,USB-350321-100
Manufacturer:
US Biological
Category:
Molekularbiologie
TBCC (tubulin folding cofactor C) belongs to the TBCC family. It is plays a role in the regulation of centrosome and Golgi apparatus positioning, with consequences on cell shape and cell migration. Cofactor C is one of four proteins(cofactors A,D,E and C) involved in the pathway leading to correctly folded b-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing b-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/b-tubulin complex, interaction with cofactor C then causes the release of b-tubulin polypeptides. Recombinant protein corresponding to aa1-346 of human TBCC, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~41.7kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMESVSCS AAAVRTGDME SQRDLSLVPE RLQRREQERQ LEVERRKQKR QNQEVEKENS HFFVATFARE RAAVEELLER AESVERLEEA ASRLQGLQKL INDSVFFLAA YDLRQGQEAL ARLQAALAER RRGLQPKKRF AFKTRGKDAA SSTKVDAAPG IPPAVESIQD SPLPKKAEGD LGPSWVCGFS NLESQVLEKR ASELHQRDVL LTELSNCTVR LYGNPNTLRL TKAHSCKLLC GPVSTSVFLE DCSDCVLAVA CQQLRIHSTK DTRIFLQVTS RAIVEDCSGI QFAPYTWSYP EIDKDFESSG LDRSKNNWND VDDFNWLARD MASPNWSILP EEERNIQWD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
