uLBP2, also known as NKG2D ligand 2, belongs to the MHC class I family. This protein is ligand for the NKG2D receptor, together with at least uLBP1 and uLBP3. uLBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of uLBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein uL16. The interaction with uL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. uL16 also causes uLBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. Source: Recombinant protein corresponding to aa26-216 of human uLBP2, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~24.3kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGRADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT FLHYDCGNKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLRDIQL ENYTPKEPLT LQARMSCEQK AEGHSSGSWQ FSFDGQIFLL FDSEKRMWTT VHPGARKMKE KWENDKVVAM SFHYFSMGDC IGWLEDFLMG MDSTLEPSAG APLAMS Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
