AKR7A3, Recombinant, Human, aa1-331, His-Tag (Aldo-keto reductase family 7, member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2, AFAR2)
Biozol Catalog Number:
USB-351070
Supplier Catalog Number:
351070
Alternative Catalog Number:
USB-351070-50,USB-351070-250
Manufacturer:
US Biological
Category:
Molekularbiologie
Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant protein corresponding to aa1-331 from human AKR7A3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~41.6kD (370aa) Specific Activity: >700pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1pmole of 1,2-Naphthoquinone presence of NADPH/minute at pH 7.0, 25C. Amino Acid Sequence: MRGSHHHHHH GQSETILGGL LSNYAAWEVA YRNRYWKEHH VAHECPNYFR GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
