AKT1, Recombinant, Human, aa1-480, His-Tag (RAC-alpha Serine/Threonine-Protein Kinase, AKT, CWS6, PKB, PRKBA, RAC, RAC-ALPHA)

Product Overview

• Article Name: AKT1, Recombinant, Human, aa1-480, His-Tag (RAC-alpha Serine/Threonine-Protein Kinase, AKT, CWS6, PKB, PRKBA, RAC, RAC-ALPHA)
• Biozol Catalog Number: USB-351071
• Supplier Catalog Number: 351071
• Alternative Catalog Number: USB-351071-50,USB-351071-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum- starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Recombinant protein corresponding to aa1-480 from human AKT1, fused to His-tag at C-terminal, expressed in Baculovirus. Uniprot/Swiss Accession: P31749 Molecular Weight: ~56.7kD (488aa) Amino Acid Sequence: MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KKQEEEEMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAG IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA LEHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 56.7
• NCBI: 001014432
• UniProt: P31749
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL)
• Form: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.