Antigen 85A, Recombinant, Mycobacterium tuberculosis, aa43-338, His-Tag (Antigen 85 complex A, Mycolytransferase Ag 85A, Fibronectin-binding protein A, Ag 85a)

Catalog Number: USB-351085
Article Name: Antigen 85A, Recombinant, Mycobacterium tuberculosis, aa43-338, His-Tag (Antigen 85 complex A, Mycolytransferase Ag 85A, Fibronectin-binding protein A, Ag 85a)
Biozol Catalog Number: USB-351085
Supplier Catalog Number: 351085
Alternative Catalog Number: USB-351085-50,USB-351085-250
Manufacturer: US Biological
Category: Molekularbiologie
Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6-monomycolate (TMM) by Antigen 85A. Recombinant protein corresponding to aa43-338 from Mycobacterium tuberculosis Antigen 85A, fused to His-tag at C-terminal expressed in insect cell. Uniprot/Accession: NP_218321.1 Molecular Weight: ~32.8kD (305aa) Amino Acid Sequence: ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 32.8
NCBI: 218321
Purity: 95% (SDS-PAGE). Endotoxin: 1EU/ug (LAL)
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.