Carbonic Anhydrase, Recombinant, E. coli, aa1-220, His-Tag (D-gluconate Kinase, thermosensitive)
Biozol Catalog Number:
USB-351121
Supplier Catalog Number:
351121
Alternative Catalog Number:
USB-351121-20,USB-351121-100
Manufacturer:
US Biological
Category:
Molekularbiologie
D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187aa protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Source: Recombinant protein corresponding to aa1-220 from E.coli idnk, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~23.4kD (210aa) Specific Activity: >80units/mg, in which one unit will convert 1umole of D-gluconate to 6-phospho-D-gluconate/minute at pH 8.0 at 37C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
