CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. Recombinant protein corresponding to aa24-92 from human CCL3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~10kD (90aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
