CD74, Recombinant, Human, aa73-232, His-Tag (Cluster of Differentiation 74, HLA class II histocompatibility antigen gamma chain Isoform b, DHLAG, HLADG)
Biozol Catalog Number:
USB-351146
Supplier Catalog Number:
351146
Alternative Catalog Number:
USB-351146-50,USB-351146-250
Manufacturer:
US Biological
Category:
Molekularbiologie
CD74, also known as HLA class II histocompatibility antigen gamma chain or HLA-DR antigens- associated invariant chain or CD74 (Cluster of Differentiation 74). The nascent MHC class II protein in the rough ER has its peptide-binding cleft blocked by the invariant chain (Ii, a trimer) to prevent it from binding cellular peptides or peptides from the endogenous pathway. The invariant chain also facilitates MHC class IIs export from the ER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a lateendosome containing the endocytosed, degraded proteins. It is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment calle CLIP which blocks peptide binding until HLA-DM binds to MHC II, releasing CLIP and allowing other peptides to bind. The stable MHC class-II is then presented on the cell surface. Source: Recombinant protein corresponding to aa73-232 from human CD74, fused to His-tag at C-terminal expressed in insect cell. Molecular Weight: ~19.05kD (166aa) Amino Acid Sequence: QQQGRLDKLT VTSQNLQLEN LRMKLPKPPK PVSKMRMATP LLMQALPMGA LPQGPMQNAT KYGNMTEDHV MHLLQNADPL KVYPPLKGSF PENLRHLKNT METIDWKVFE SWMHHWLLFE MSRHSLEQKP TDAPPKESLE LEDPSSGLGV TKQDLGPVPM HHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
