Gstp1 also known as Glutathione S-transferase P 1. Gstp1 is a superfamily of ubiquitously expressed cytosolic, mitochondrial and membrane-associated enzymes involved in a range of detoxification processes. Also Increased Gstp1 levels were consistently associated with reduced Cdk5 activity. Gstp1 directly inhibits Cdk5 by dislodging p25/p35, and indirectly by eliminating oxidative stress. Source: Recombinant protein corresponding to aa1-210 from mouse Gstp1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~26kD(233aa) Specific Activity: >40 units/mg, and is defined as the amount of enzyme that conjugate 1umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione/minute at pH 6.5 at 25C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPPYTIV YFPVRGRCEA MRMLLADQGQ SWKEEVVTID TWMQGLLKPT CLYGQLPKFE DGDLTLYQSN AILRHLGRSL GLYGKNQREA AQMDMVNDGV EDLRGKYVTL IYTNYENGKN DYVKALPGHL KPFETLLSQN QGGKAFIVGD QISFADYNLL DLLLIHQVLA PGCLDNFPLL SAYVARLSAR PKIKAFLSSP EHVNRPINGN GKQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
