HARS, Recombinant, Human, aa1-509, His-Tag (Histidyl-tRNA synthetase, cytoplasmic, HRS, HisRS, Histidine--tRNA ligase)

Product Overview

• Article Name: HARS, Recombinant, Human, aa1-509, His-Tag (Histidyl-tRNA synthetase, cytoplasmic, HRS, HisRS, Histidine--tRNA ligase)
• Biozol Catalog Number: USB-351276
• Supplier Catalog Number: 351276
• Alternative Catalog Number: USB-351276-10,USB-351276-50,USB-351276-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant protein corresponding to aa1-509 from human HARS, fused to His-tag at N-terminal expressed in E. coli. Accession Number: NP_002100 Molecular Weight: ~59.8kD (532aa) Amino Acid Sequence: MAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 59.8
• NCBI: 002100
• UniProt: P12081
• Purity: 95% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.