Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Source: Recombinant protein corresponding to aa49-162 from human Interleukin-15 fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~13.9kD (123aa) Biological Activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells, the ED50 for this effect is <2.5ng/ml. Activity Assay: 1. Cell line: CTLL2 (mouse cytotoxic Tcell) 2. Maintenance Condition: 10% FBS RPMI 1640 with hIL2 3. Assay Medium: 10% FBS RPMI 1640 without hIL2 4. Cell Density: 2x10e4 cells/well (96 well plate, final volume 100ul) 5. Starvation: 24hr, Assay medium 6. Incubation Time: 24hr (after sample treatment) 7. Concentration Range: 0.039-40ng/ml 8. Detection Method: MTT assay Amino Acid Sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH Endotoxin: ~1EU/1ug of protein (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
