KPNA2, Recombinant, Human, aa1-529, His-Tag (Importin Subunit alpha-2, IPOA1, QIP2, SRP1alpha)

Product Overview

• Article Name: KPNA2, Recombinant, Human, aa1-529, His-Tag (Importin Subunit alpha-2, IPOA1, QIP2, SRP1alpha)
• Biozol Catalog Number: USB-351332
• Supplier Catalog Number: 351332
• Alternative Catalog Number: USB-351332-50,USB-351332-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Karyopherin alpha2 (KPNA2) is a member of the karyopherin alpha family. This protein plays a key role in the nuclear import of proteins with a classical nuclear localization signal (NLS). KPNA2 bound in vitro specifically and directly to substrates containing either a simple or bipartite NLS motif. It promoted docking of import substrates to the nuclear envelope and together with recombinant human Ran reconstituted complete nuclear protein import. Recombinant protein corresponding to aa1-529 from human KPNA2, fused to His-Tag at N-terminal expressed in E. coli. Swiss/Uniprot: P52292 Molecular Weight: ~60.5kD (553aa) Amino Acid Sequence: MSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQ MLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNII RAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWAL GNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTL VRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDE QTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKE AVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEE CGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 60.5
• NCBI: 05978
• UniProt: P52292
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol.