KRT20 (Keratin type I Cytoskeletal 20, Keratin, type I Cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21), Mouse
Biozol Catalog Number:
USB-351337
Supplier Catalog Number:
351337
Alternative Catalog Number:
USB-351337-50,USB-351337-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, FC, IF, IHC, WB
Immunogen:
Recombinant protein corresponding to human KRT20, aa1-424, expressed in E. coli.
Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:3000 Immunocytochemistry/Immunofluorescence: 1:200 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
