METTL1, Recombinant, Human, aa1-276, His-Tag (Methyltransferase like 1, TRM8, YDL201w)
Biozol Catalog Number:
USB-351368
Supplier Catalog Number:
351368
Alternative Catalog Number:
USB-351368-50,USB-351368-250
Manufacturer:
US Biological
Category:
Molekularbiologie
METTL1 is known as tRNA (guanine-N(7)-)-methyltransferase which belongs to the methyltransferase superfamily. METTL1 shows high sequence similarity to yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. This protein contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphylation. It catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Recombinant protein corresponding to aa1-276 from human METTL1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~33.6kD (296aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
