Nme2 also known as nucleoside diphosphate kinaseB is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. Nme2 is a ubiquitous enzyme that catalyses phosphorylation of nucleoside 5 -diphosphate (NDP) to the corresponding triphosphate (NTP), following a Ping-Pong mechanism which includes the formation of a phosphohistidine intermediate. Source: Recombinant protein corresponding to aa1-152 from rat Nme2, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~19.7kD (175aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANLERT FIAIKPDGVQ RGLVGEIIKR FEQKGFRLVA MKFLRASEEH LKQHYIDLKD RPFFPGLVKY MNSGPVVAMV WEGLNVVKTG RVMLGETNPA DSKPGTIRGD FCIQVGRNII HGSDSVESAE KEIGLWFKPE ELIDYKSCAH DWVYE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
