NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Source: Recombinant protein corresponding to aa22-169 from human NME3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~19.1kD (169aa) Specific Activity: >150units/mg, and is defined as the amount of enzyme that convert 1umole each of ATP and TDP to ADP and TTP/minute at pH 7.5 at 25C in a couple system with PK/LDH. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
