CST complex subunit STN1, also known as OBFC1, is a component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence- independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Recombinant protein corresponding to aa1-368 from human OBFC1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~44.5kD(391aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQPGSSR CEEETPSLLW GLDPVFLAFA KLYIRDILDM KESRQVPGVF LYNGHPIKQV DVLGTVIGVR ERDAFYSYGV DDSTGVINCI CWKKLNTESV SAAPSAAREL SLTSQLKKLQ ETIEQKTKIE IGDTIRVRGS IRTYREEREI HATAYYKVDD PVWNIQIARM LELPTIYRKV YDQPFHSSAL EKEEALSNPG ALDLPSLTSL LSEKAKEFLM ENRVQSFYQQ ELEMVESLLS LANQPVIHSA CSDQVNFKKD TTSKAIHSIF KNAIQLLQEK GLVFQKDDGF DNLYYVTRED KDLHRKIHRI IQQDCQKPNH MEKGCHFLHI LACARLSIRP GLSEAVLQQV LELLEDQSDI VSTMEHYYTA F Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
