PGM1 is also known as Phosphoglucomutase-1 isoform 1. PGM1 belongs to the phosphohexose mutase family. There are several PGM isozymes, which are catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. Defects in PGM1 are the cause of glycogen storage disease type 14. Source: Recombinant protein corresponding to aa1-562 from human PGM1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~63.8kD (585aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVKIVTV KTQAYQDQKP GTSGLRKRVK VFQSSANYAE NFIQSIISTV EPAQRQEATL VVGGDGRFYM KEAIQLIARI AAANGIGRLV IGQNGILSTP AVSCIIRKIK AIGGIILTAS HNPGGPNGDF GIKFNISNGG PAPEAITDKI FQISKTIEEY AVCPDLKVDL GVLGKQQFDL ENKFKPFTVE IVDSVEAYAT MLRSIFDFSA LKELLSGPNR LKIRIDAMHG VVGPYVKKIL CEELGAPANS AVNCVPLEDF GGHHPDPNLT YAADLVETMK SGEHDFGAAF DGDGDRNMIL GKHGFFVNPS DSVAVIAANI FSIPYFQQTG VRGFARSMPT SGALDRVASA TKIALYETPT GWKFFGNLMD ASKLSLCGEE SFGTGSDHIR EKDGLWAVLA WLSILATRKQ SVEDILKDHW QKYGRNFFTR YDYEEVEAEG ANKMMKDLEA LMFDRSFVGK QFSANDKVYT VEKADNFEYS DPVDGSISRN QGLRLIFTDG SRIVFRLSGT GSAGATIRLY IDSYEKDVAK INQDPQVMLA PLISIALKVS QLQERTGRTA PTVIT Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
