TEK, also known as angiopoietin-1 receptor isoform 2, is cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4. It regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton. This protein effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Recombinant protein corresponding to aa23-746 from mouse TEK fused to His-tag at C-terminal expressed in insect cell. Molecular Weight: ~81.6kD (730aa) Amino Acid Sequence: AMDLILINSL IRTMKMRQQA FTRLIVRRCE WRGLQCNEAC MTLVKPDGTV DGPIKSKKLF PIFTNSEDEF TAMVSWTIVD GKMLHHHHHH PLVSDAETSL TCIASGWHPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGQAIR SFLPATLTMT VDRGDNVNIS FKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA AQKWGPDCSR PCTTCKNNGV CHEDTGECIC PPGFMGRTCE KACEPHTFGR TCKERCSGPE GCKSYVFCLP DPYGCSCATG PSGYYGPDCK LRCHCTNEEI CDRFQGCLCS QGWQGLQCEK EGRPRMTPQI EDLPDHIEVN SGKFNPICKA SGWPLPTSEE LQPNDFNYTD RFSVAIFTVN RVLPPDSGVW VCSVNTVAGM VEKPFNISVK VLPEPLHAPN VIDTGHNFAI INISSEPYFG YKPVNQAWKY IEVTNEIFTL NYLEPRTDYE LCVQLARPGE GGEGHPGPVR RFTTASIGLP PPRGLSLLPK SQTALNLTWQ YVEVERRSLQ TTSDQQNIKV PGNLTSVLLS NLVPREQYTV RARVNTKAQG EWSEELRAWT LSDILPPQPE NIKISNITDS GYSISSIIIR YKVQGKNEDQ HIDVKIKNAT VTQYQLKGLE PETTYHVDIF AENNIGSSNP AFSHELRTLP HSPASADLGG Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
