TIGAR, Recombinant, Human, aa1-270, His-Tag (TP53-induced glycolysis and apoptosis regulator)
Biozol Catalog Number:
USB-351521
Supplier Catalog Number:
351521
Alternative Catalog Number:
USB-351521-50,USB-351521-250
Manufacturer:
US Biological
Category:
Molekularbiologie
TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270aa protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. Source: Recombinant protein corresponding to aa1-270 from human TIGAR, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~32.6kD (294aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
