TRPT1, also known as tRNA 2-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2-phosphate from ligated tRNA to NAD to produce ADP-ribose 1-2 cyclic phosphate. Recombinant protein corresponding to aa1-253 from human TRPT1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~30.3kD (277aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
