The Agrin (AGRN) BioAssay(TM) ELISA Kit (Rat) is sandwich ELISA intended for the quantitative determination of rat agrin (AGRN) concentrations in serum, plasma, tissue homogenates and cell lysates. Sensitivity: 19.5pg/ml Range: 78-5000pg/ml Intra-assay Precision: %CV <8 Inter-assay Precision: %CV <10 Specificity: This assay has high sensitivity and excellent specificity for detection of rat AGRN. No significant cross-reactivity or interference between rat AGRN and analogues was observed. Test Principle: Antibody specific for AGRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGRN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGRN bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells Standard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
