This protein is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. Intended Use: For the quantitative determination of human granulocyte-macrophage colony stimulating factor (GM-CSF) concentrations in serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 15.6-1000pg/ml Sensitivity: 3.9pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of human GM-CSF. No significant cross-reactivity or interference between human GM-CSF and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: 351644A: Microtiter strips, 1x48 wells or 1x96 wells *351644B: Standard (Freeze dried), 2x1vial or 2x 1vials 351644C: Biotin antibody, 100X, 1x60ul or 1x120ul 351644D: HRP avidin, 100X, 1x60ul or 1x120ul 351644E: Biotin antibody Diluent, 1x7.5ml or 1x15ml 351644F: HRP avidin Diluent, 1x7.5ml or 1x15ml 351644G: Sample Diluent, 1x25ml or 1x50ml 351644H: Wash Buffer, 25X, 1x10ml or 1x20ml 351644K: TMB Substrate, 1x5ml or 1x10ml 351644L: Stop Solution, 1x5ml or 1x10ml Storage and Stability: Store *351644B powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
