Hemoglobin, Free (f-Hb) BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Hemoglobin, Free (f-Hb) BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-351646
• Supplier Catalog Number: 351646
• Alternative Catalog Number: USB-351646-48, USB-351646-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Sample Type: Serum, plasma Intended Use: For the quantitative determination of mouse free hemoglobin (f-Hb) concentrations in serum, plasma. Sensitivity: 0.177ug/ml Range: 0.781-50ug/ml Specificity: This assay has high sensitivity and excellent specificity for detection of mouse f-Hb. No significant cross-reactivity or interference between mouse f-Hb and analogues was observed. Test Principle: This BioAssay(TM) employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hb. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated Hb. The competitive inhibition reaction is launched between with HRP-conjugated Hb and Hb in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of f-Hb in the sample. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate, 1x96 wells Standard (Freeze dried), 2x vials HRP-conjugate (100X), 1x60ul HRP-conjugate Diluent, 1x10ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.